ID   SPBC1215 standard; DNA; FUN; 6490 BP.
XX
AC   AL096846;
XX
DE   S.pombe chromosome II cosmid c1215.
XX
KW   SURF-family protein; COX complex biogenesis; DEC1 homologue;
KW   mitochondrial inheritance; actin cytoskeleton organisation.
XX
OS   Schizosaccharomyces pombe (yeast)
OC   Eukaryota; Plantae; Thallobionta; Eumycota; Hemiascomycetes;
OC   Endomycetales; Saccharomycetaceae.
XX
RN   [1]
RP   1-6490
RA   Lyne M.H., Rajandream M.A., Barrell B.G., Seeger K., Quail M., Harris D.;
RT   ;
RL   Submitted (16-JUL-1999) to the EMBL/GenBank/DDBJ databases.      
RL   European Schizosaccharomyces genome sequencing project,
RL   Sanger Centre, The Wellcome Trust Genome Campus, Hinxton, 
RL   Cambridge CB10 1SA, E-mail: barrell@sanger.ac.uk and  
XX
CC   Notes:
CC
CC   Details of yeast sequencing at the Sanger Centre are available on
CC   the World Wide Web. 
CC   (URL, http://www.sanger.ac.uk/Projects/S_pombe/)
CC   During 1995 to 1996 about 66% of S. pombe chromosome 1 was sequenced 
CC   by the Sanger Centre.  The sequencing of the S. pombe genome is now 
CC   being continued with funding from The European Commission.  
CC   Fourteen European sequencing laboratories, including the Sanger Centre,  
CC   are participating in the project.
CC
CC   Protein coding regions (CDS) have been predicted with the help 
CC   of computer analysis using the Genefinder program in PomBase
CC   (an ACEDB database) with additional predictions for the
CC   branch-acceptor sites supplied by the program Sp3splice.
CC   CAUTION: It is possible that for any individual CDS we may have
CC   underestimated or overestimated the number of introns/exons or
CC   we may not have chosen the correct splice donor/acceptor sites.
CC   
CC   CDS are numbered using the following system eg SPBC25H2.01c.
CC   SP (S. pombe), B (chromosome 2), c25H2 (cosmid name),
CC   .01 (first CDS), c (complementary strand).
CC
CC   The more significant matches with motifs in the PROSITE
CC   database are also included but some of these may be fortuitous.
CC
CC   The length in codons is given for each CDS.
CC   
CC   IMPORTANT: This sequence MAY NOT be the entire insert of
CC   the sequenced clone.  It may be shorter because we only
CC   sequence overlapping sections once, or longer, because we
CC   arrange for a small overlap between neighbouring submissions.
CC
CC   Cosmid c1215 is overlapped at the 5' end by cosmid c1750, 
CC   EMBL entry SPAB4534, accession number AB004534, and at the 3' 
CC   end by cosmid c83, EMBL entry SPBC83, accession number AL035536.
XX
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..6490
FT                   /organism="Schizosaccharomyces pombe"
FT                   /strain="972h-"
FT                   /chromosome="II"
FT                   /clone="cosmid c1215"
FT                   /map="IIL"
FT   misc_feature    complement(1..82)
FT                   /note="nominal overlap with cosmid SPAB4534 (c1750) S.
FT                   pombe chromosome 2"
FT   tRNA            36..107
FT                   /note="tRNA Thr anticodon TGT, Cove score 78.51"
FT   rRNA            415..581
FT                   /note="SPRG5SD K00771 Yeast (s.pombe) 5s rrna gene and
FT                   flanks"
FT   CDS             join(1501..1760,1844..2456)
FT                   /fasta_file="fasta/gf.tab.seq.00001.out"
FT                   /note="SPBC1215.01, len:290, SIMILARITY:Saccharomyces
FT                   cerevisiae, YG2X_YEAST, hypothetical 45.1 kd protein in
FT                   clb6-spt6 intergenic region, (389 aa), fasta scores: opt:
FT                   385, E():2.8e-19, (29.0% identity in 314 aa)"
FT                   /gene="SPBC1215.01"
FT                   /product="putative SURF-family protein"
FT                   /colour=7
FT                   /label=SPBC1215.01
FT   misc_feature    1761..1766
FT                   /note="gtacgt, splice donor sequence"
FT                   /colour=6
FT   misc_feature    1827..1843
FT                   /note="ctaacataatcacttag, splice branch and acceptor"
FT                   /colour=6
FT   CDS             complement(join(2495..2602,2663..2881,2953..4071,4111..4372,4413..4849,4889..5007,5251..5389,5434..5466))
FT                   /fasta_file="fasta/gf.tab.seq.00002.out"
FT                   /note="SPBC1215.02c, len:811, SIMILARITY:Saccharomyces
FT                   cerevisiae, DEC1_YEAST, dec1 protein, (796 aa), fasta
FT                   scores: opt: 184, E():0.00014, (23.4% identity in 577 aa)"
FT                   /gene="SPBC1215.02c"
FT                   /product="similar to yeast DEC1 mitochondrial inheritance
FT                   and actin cytoskeleton organisation protein"
FT                   /colour=7
FT                   /label=SPBC1215.02c
FT   misc_feature    complement(2603..2612)
FT                   /note="ctaataatag, splice branch and acceptor"
FT                   /colour=6
FT   misc_feature    complement(2657..2662)
FT                   /note="gtacgt, splice donor sequence"
FT                   /colour=6
FT   misc_feature    complement(2882..2896)
FT                   /note="ctaaccgaattatag, splice branch and acceptor"
FT                   /colour=6
FT   misc_feature    complement(2947..2952)
FT                   /note="gtaagt, splice donor sequence"
FT                   /colour=6
FT   misc_feature    complement(4072..4085)
FT                   /note="ttaactcgtcgtag, splice branch and acceptor"
FT                   /colour=6
FT   misc_feature    complement(4105..4110)
FT                   /note="gtaagt, splice donor sequence"
FT                   /colour=6
FT   misc_feature    complement(4373..4387)
FT                   /note="ctaacttctccatag, splice branch and acceptor"
FT                   /colour=6
FT   misc_feature    complement(4407..4412)
FT                   /note="gtaagt, splice donor sequence"
FT                   /colour=6
FT   misc_feature    complement(4850..4861)
FT                   /note="ctaactcctcag, splice branch and acceptor"
FT                   /colour=6
FT   misc_feature    complement(4883..4888)
FT                   /note="gtaaga, splice donor sequence"
FT                   /colour=6
FT   misc_feature    complement(5008..5021)
FT                   /note="ttaactatttgaag, splice branch and acceptor"
FT                   /colour=6
FT   misc_feature    complement(5245..5250)
FT                   /note="gtaagt, splice donor sequence"
FT                   /colour=6
FT   misc_feature    complement(5390..5405)
FT                   /note="ttaacatcctttttag, splice branch and acceptor"
FT                   /colour=6
FT   misc_feature    complement(5428..5433)
FT                   /note="gtaagt, splice donor sequence"
FT                   /colour=6
FT   misc_feature    6387..6490
FT                   /note="nominal overlap with cosmid SPBC83 S. pombe
FT                   chromosome 2"
SQ   Sequence 6490 BP; 2097 A; 1138 C; 1138 G; 2117 T; 0 other;
     tatatataat ttaataaata cattccgacg atactgcctc tatggcttag tggtacagca        60
     tcgcacttgt aatgcgaaga tccttggttc gattccgagt ggaggcatat acattatatt       120
     atattctttt tcatgcggaa aaaagatttc aaatttttgg gtatgatatt aatatgactg       180
     taacgttaat agcaaagtga gtgttaataa tgataaaata gcagcaaaat ctcttttccg       240
     agtaagacgt tttccagtct aaatttggag tctgcagttg tttcgcaatt cttaatgtat       300
     ggttatacta aatacaaact ttaaagctct gatttatgtt tgcaataaac taaaataaaa       360
     gcacaaaaac ctttacccat taatttcaaa caacttataa actaccggta aacttttttt       420
     ctaaccttta taatttataa actagaatgt ttaatgtcta cggccatacc taggcgaaaa       480
     caccagttcc cgtccgatca ctgcagttaa gcgtctgagg gcctcgttag tactatggtt       540
     ggagacaaca tgggaatccg gggtgctgta ggctattttt ttatatccgt ctttcttact       600
     acttgcctaa caagtcatga tgtactctca aaatatgttt gcatgccttg taatattggt       660
     tatggatagc tccttctgga cttgatcttt tgtagccaag aacaatgggt atagactctg       720
     accttgtgat gttgtagcca cagattataa taggtatttt caagtacagt aacaaaaatc       780
     ttctagtttt tttttagaaa ggatacacca agtataagca aattcaggaa ttgttgatta       840
     aactgtcaac ttcggtaaaa ctttgggcat aagtagtgtg ggagcaagtt taactaaaat       900
     tctattcaga tgtcgaatcc aaaccgctaa ttttgctcaa ctagcttttc ataaaaacca       960
     attcatagtt tcatactaat aaagacgatt gtttacttta aaacatacgt cgtaagaaca      1020
     tatattgctt tatcgaaaga taacaaatgt taagctatta tattatttaa ctatagcgca      1080
     gatttcgctt cctttactta aaaaagacat gtgacttgta gaagcttgga gtgaatacgc      1140
     aaaggtacct acttagacat tcgcgtctct cttagctgtc aacatcaaca aactggcccc      1200
     gtattgaaca gtatcttact tgtcgaagga tttgactaag aaaattttat ttctttatag      1260
     caatattccg ttttcgctta gaagattcta gtcaattgcc ctattctact tacgctttac      1320
     agtagtatca gaagacctga gtgggatttt gctgctagta gaggccattc aagttaactc      1380
     cgttttgcaa cattttaaaa gtttttgaat tgaatataaa tatcaattgt ttgattcctt      1440
     ttaggattta atcttttctt tttatttttg tttcgattga atcttggatt cctgtctatc      1500
     atgttttggt ggaaaagtgc tactaaattc acattctcaa agcgtggacc gtgtgtcttt      1560
     cgctatttga gtactcttga aggaacaact gtgaggccta aaaaaaataa atttttagtt      1620
     ggattgcttt ctgccgttcc aattgtcacg tttgctttag gaacttggca ggtaaagcga      1680
     cgagaatgga aaatgggtat catcaataca ctcacggaaa ggcttcaaca gcccgcaatt      1740
     ttattaccga aaactgttac gtacgttaag ttaacatata cacaaattgc acgttttgca      1800
     attgaactgt cgttttttac attaagctaa cataatcact tagagagcaa gatacaaaaa      1860
     aacttgagtg gactagggtt ttgcttcgtg gtgtgttttg tcacgaccaa gaaatgttgg      1920
     tcggtccaag aacgaaggaa ggccaacctg gctatcacgt agtaacccca tttattttag      1980
     acgatgggcg tcgaatttta gtcaacagag gatggattgc tcgatcattt gctgaacagt      2040
     cttctcgaga tcctagttct ttacctaaag gtccagtggt cattgaaggt cttttgagac      2100
     aacatactga taagccaaga tttatgatga agaatgagcc tgaaaaaaat tctttttact      2160
     tcttaaatgt tcgtgagttt gcacaattga aaggaactct ccccattttg ataacagaac      2220
     tacaaccatc gcttacaccg ttgcaagaag ccgatcatgt taagagaggc ttgcctcttg      2280
     gtcatcctct aaaagttgaa attttcaaca gtcatacaga atatattatc acttggtatt      2340
     ctctaagtgt ggtatcagct ataatgcttt acgtctattt taagagaggt tcaggcacat      2400
     cttctctgaa ttctgcatac gaaagaagca agattctaaa caacaaacga ttataaaaaa      2460
     ttttcatatt tataagtttc taaatattat ctacctaaaa ttttacaaat tttggaagct      2520
     tgcttactgc gtccgtcgtt tgaatgtatg aatcgatcat tccttcacca acttgttttg      2580
     caaacttcgg atcgtaagga acctattatt aggaagttag ttctcatgcc tataatttta      2640
     atgctctata atcatcacgt acctgagttt cagataaatt gctcagccaa gagtttgaca      2700
     acaattctga tacatatttt ctggcggcct tctttttatg cttcgtaatt ccagagatac      2760
     ttcctaattt gttactgctg atgtttttaa gaagttggta ttgtctggta aattcttttt      2820
     tcttggtagc tgacacgtga tataggaagc tgtttagaca agtgataagg tcatttatga      2880
     tctataattc ggttagttta gaaattttta tatcattttt tttaaaaaaa aaaaccagtt      2940
     tggtaaactt acttcagtgt actttgtcaa ttgacttaaa ggcgtggagt tctcataatc      3000
     gaatgactca attaaatttt caatcgtttc gaacgaggaa ttttcgtagt ctccgttctt      3060
     gacctttacc gaaagtaatc ccagttggat caataatttc atatgaacaa tttcttccga      3120
     agttaattgc ttcgataaat cattgttttc gcacaaaact tccatttcct tagcacttaa      3180
     aacggccttt tcaaaatcgc cgtttactat gctgtcttga acgagagaat gaccaatgac      3240
     agtcaaatgg atccaaagcg tgtccggttt aggcgaattt cttaaacttt cttcaactgt      3300
     tggcaattta tcagatccat aatctgcaaa gacctttaaa tctcgattat ctttcggaga      3360
     tgaacatttg ggaagatact gtttgggtgg tttaaaagcg gtaagataat gtatcctagc      3420
     ccgctcgacc aaagaaatgc ttttccaggt actgtggtct aaacgggatc ggaaattacg      3480
     catatcttcg atttgagagt aagcaccatc ttcataagcc atagaaatca tctctggtgt      3540
     ttcaaactca ttcgaaccat atattttgag acttgagtta atatagtgag aagtaacaga      3600
     agatggataa taggtagtag cacgagtcaa gagataatga tcaagggtat cgttttgaat      3660
     ttgcttaatg gacatagtgt cgtaaacttt agcagcagca gggaagcctc catcaagaag      3720
     aagatataaa cgaataaggg gtagctttaa atgaaagtta tgttgactat aagtaatgcc      3780
     tttttctaaa agacagattg cgtcaaaaat taaagcttgt ttttcggcag gttttaaatc      3840
     cttattcccc tcccacatat atatcaatga atgaactgct aataacaaag cttcataacc      3900
     gtgcgtaaag tcagtgggta ataagccttt acttaaagac aaaccctttt caaatgcgac      3960
     gaagcatcta cgcacgtaat cgacaaccga ttctgcagta aacgactcaa agagcaaaaa      4020
     atggatcttc agaagcaaaa cctcagcata taatttatcg actttttgag actacgacga      4080
     gttaatattg aaagatgaat aaatacttac ttcattggat tcacctagat cagccaactt      4140
     aaatgcatct aataaccggt gttgtgcatc aacattcagc ttcaatagat aaggtctcaa      4200
     atcttcaaaa acaattggtt tcatatacag ctttttgata tagccaagta atgctgattc      4260
     gtgttcttca ggaaaaaacc gtgcgctggc ttcaatccaa agtaaatgaa gatttctttt      4320
     tgtgctgctg gttgacaacg ctttcaatat acaatccttt aatgggacta atctatggag      4380
     aagttagtaa taatgcaaaa aaaaaaactt actttgaatc atcattggaa gcactatcca      4440
     gcaaagcttt gcaaaccttc caatcagtat tcccagtttg gaaaagagaa agcgaaaacg      4500
     tgaataaaga atcccaacgc gcacaggaag caagcaattc aagcttcctc aaaagaaggt      4560
     cggcgtcagc atcaacaaag cgatcagcat cttgatgtat taaagcatct aacgctctgt      4620
     ccttgtctcc cactaaaagc aacacgtcta aataaagatg aaactcctca caagaatcga      4680
     tataacctgt tggtttttcg aaaatgagtt tagcagtttt ttcagctagt gctttcagca      4740
     aacgttgttc aacctcattt tccgactttt tggacaaaag atacaaagat gagataaccc      4800
     ataaggtatg tttcctagaa ggaaagttct tttgcaattc tacagcagcc tgaggagtta      4860
     gtaaaaaaaa aacaaattaa gatcttacct ttctttggtg agacaatgat ttgatgcgaa      4920
     tagatgcttt gaagtaagct aataagtttt tttcctgctt tccataagtt tgcaagaact      4980
     tttcccaaaa tacaaacgat tcttctcctt caaatagtta ataacttaaa agtaagaggg      5040
     taaaaacaag tccattgaga aataaaacgt tgcacttgaa tcatcaagct acgaaaagaa      5100
     caaagatcgt ttttcttaag caaagtttaa ccgatcaaat aactaaagcc tttatctcaa      5160
     ggaaatagta gaatattcaa aataaaaaat acgaccaaag tgctatcaac atttatgttc      5220
     atctgttcaa tagaacctaa ttatacttac ccttcttttg atcatcataa acagcttgta      5280
     taatgtctaa aagttccaag ctgttgatgg gagtcgattt taacggttct aaaagtgcta      5340
     gagcctcggg accacgtcct gcctgagcca aggataacgc tgaataaacc taaaaaggat      5400
     gttaacagct aaaaacataa ctcgattact tacaattgtt gattctttac tcccagaacg      5460
     acgcattgta gtaggcaatg aaattaaagc ccaaacagta gtcagaagtt ggtaggtgtt      5520
     gcagtcaaat tattattcta cagaggagaa tattatagcc agcgtggtag aatctggata      5580
     tatatctact gcaaaagtgt aattgcattg gtttaaaggg tatactatgg ttaagtaata      5640
     tattcacagc tgtacaattt acagtcataa ctaaaacttc cttaagccgt aaagaaatac      5700
     ctggtgttgt aaaatttgtt gtatatccac ggcatggtca tataatgtga ttttgtgctc      5760
     aaataaatat aaaatatgca taatttttgt acatttaatt tgagaaaccc atcttttgtt      5820
     gagaggctgt caatgaatag cagtttcatt gaaaagcagc gggatgacca gaaaagtatt      5880
     ttacaatggc aagggagtag aaagctagcg taatattcag aaagctaggt aattgagcaa      5940
     tcctttaatt cattgctaag catgctaggt aaacgcagta aacctttcag ttttcattta      6000
     ggtataaggc tgtttaatga gtatctccac taaatttaaa gatcaaaact cagtatcaat      6060
     tcttaaaagt tttattttat ttaataatca tatacttctc ataatctttc aattttttcc      6120
     ccattttgat gatattttta ttaatcctac agtaagctct atgatatcgt tattcttcaa      6180
     ataggctggt cagcacgtgg acggtgttac ttatcgttaa ataaatcgta ctaaggaggt      6240
     gcgatgtaaa tgatatgctt gtcaagtatt aactgctctc caccaaccgc cggtttaact      6300
     gattattgtt gaaaagcgca gacgaagttt agagaattac tagcgtattt taaatttaat      6360
     caacggacta ttttttattc ctttgagatc cgactttatc gctttgcttc taattttcca      6420
     aaattcagtc tatctacgcg atccagccct gtttgcgtaa atttcatatt atttttcttt      6480
     aaacgtttgg                                                             6490
